Chromosome Research

, Volume 4, Issue 5, pp 365–371

Genomic instability in MycER-activated Rat1A-MycER cells


    • Manitoba Institute of Cell Biology
  • Monika Fluri
    • Basle Institute for Immunology
  • David Siwarski
    • Molecular Genetics Section, Laboratory of GeneticsNCI/NIH
  • Konrad Huppi
    • Molecular Genetics Section, Laboratory of GeneticsNCI/NIH
Technical Viewpoint

DOI: 10.1007/BF02257272

Cite this article as:
Mai, S., Fluri, M., Siwarski, D. et al. Chromosome Res (1996) 4: 365. doi:10.1007/BF02257272


The deregulated expression of c-Myc protein is associated with the non-random locus-specific amplification of the dihydrofolate reductase (DHFR) gene. This study was performed to determine whether additional chromosomal aberrations occur when c-Myc protein levels are up-regulated for prolonged periods. To this end, we have used Rat1A-MycER cells, which allow the experimental regulation of Myc protein levels. We examined the genomic stability of Rat1A-MycER cells cultivated in either the absence or the presence of estrogen, which reportedly activates the chimeric MycER protein in these cells. Following prolonged periods of MycER activation, Rat1A-Mycer cells exhibited irreversible chromosomal aberrations. The aberrations included numerical changes, chromosome breakage, the formation of circular chromosomal structures, chromosome fusions, and extrachromosomal elements.

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© Rapid Science Publishers 1996