Journal of Biomedical Science

, Volume 11, Issue 1, pp 117–126

Early detection of antibodies against various structural proteins of the SARS-associated coronavirus in SARS patients

Authors

  • Ho-Sheng Wu
    • Graduate Institute of Life SciencesNational Defense Medical Center
    • Division of Laboratory Research and DevelopmentCenter for Disease Control
    • Department of HealthInstitutes Biological Chemistry
  • Yueh-Chun Hsieh
    • Department of HealthInstitutes Biological Chemistry
  • In-Jen Su
    • Division of Laboratory Research and DevelopmentCenter for Disease Control
  • Ting-Hsiang Lin
    • Division of Laboratory Research and DevelopmentCenter for Disease Control
  • Shu-Chun Chiu
    • Division of Laboratory Research and DevelopmentCenter for Disease Control
  • Yu-Fen Hsu
    • Division of Laboratory Research and DevelopmentCenter for Disease Control
  • Jih-Hui Lin
    • Division of Laboratory Research and DevelopmentCenter for Disease Control
  • Mei-Ching Wang
    • Division of Laboratory Research and DevelopmentCenter for Disease Control
  • Jeou-Yuan Chen
    • Biomedical SciencesAcademia Sinica
  • Pei-Wen Hsiao
    • BioAgricultural SciencesAcademia Sinica
  • Geen-Dong Chang
    • Graduate Institute of Biochemical SciencesNational Taiwan University
  • Andrew H. -J. Wang
    • Department of HealthInstitutes Biological Chemistry
  • Hsien-Wei Ting
    • Department of HealthInstitutes Biological Chemistry
  • Chih-Ming Chou
    • Department of BiochemistryTaipei Medical University
  • Chang-Jen Huang
    • Institute of Biological ChemistryAcademia Sinica
    • Department of HealthInstitutes Biological Chemistry
Original Paper

DOI: 10.1007/BF02256554

Cite this article as:
Wu, H., Hsieh, Y., Su, I. et al. J Biomed Sci (2004) 11: 117. doi:10.1007/BF02256554

Abstract

Severe acute respiratory syndrome (SARS), a new disease with symptoms similar to those of atypical pneumonia, raised a global alert in March 2003. Because of its relatively high transmissibility and mortality upon infection, probable SARS patients were quarantined and treated with special and intensive care. Therefore, instant and accurate laboratory confirmation of SARS-associated coronavirus (SARS-CoV) infection has become a worldwide interest. For this need, we purified recombinant proteins including the nucleocapsid (N), envelope (E), membrane (M), and truncated forms of the spike protein (S1–S7) of SARS-CoV inEscherichia coli. The six proteins N, E, M, S2, S5, and S6 were used for Western blotting (WB) to detect various immunoglobulin classes in 90 serum samples from 54 probable SARS patients. The results indicated that N was recognized in most of the sera. In some cases, S6 could be recognized as early as 2 or 3 days after illness onset, while S5 was recognized at a later stage. Furthermore, the result of recombinant-protein-based WB showed a 90% agreement with that of the whole-virus-based immunofluorescence assay. Combining WB with existing RT-PCR, the laboratory confirmation for SARS-CoV infection was greatly enhanced by 24.1%, from 48.1% (RT-PCR alone) to 72.2%. Finally, our results show that IgA antibodies against SARS-CoV can be detected within 1 week after illness onset in a few SARS patients.

Key Words

SARS-CoV infection Western blotting Recombinant proteins Antibody response

Copyright information

© National Science Council 2004