Deoxyribonuclease-Inhibitory antibodies in systemic lupus erythematosus
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Deoxyribonucleases (DNases) are key enzymes for digesting DNA. Abnormalities in the function of these enzymes may contribute to the development of anti-DNA antibodies in systemic lupus erythematosus (SLE). In this study, we used bovine DNase 1-coated ELISA plates to screen anti-DNase antibodies in SLE patients. About 62% of the sera of SLE patients (63/101) were positive for anti-DNase antibodies compared to only 8% of normal controls (8/98). A positive correlation was also found between the concentrations of anti-DNase and anti-DNA antibodies in sera of SLE patients. Affinity-purified anti-DNase immunoglobulin G (IgG) from pooled sera of SLE patients bound to bovine DNase as well as DNA. A synthetic peptide, corresponding to the catalytic site of DNase, was able to completely inhibit the binding of anti-DNase IgG to DNase. In addition to bovine DNase, the anti-DNase IgG also bound to and inhibited the enzymatic activities of DNase present in streptococcal supernatants and human urine. Immunization of lupus-prone NZB/NZW mice with bovine DNase enhanced the production of anti-DNase and DNA antibodies, and accelerated the occurrence of proteinuria. Taken together, these results suggest that DNase-inhibitory antibodies which recognize a conserved epitope near the catalytic site of DNase may act in the pathogenesis of SLE.
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- Deoxyribonuclease-Inhibitory antibodies in systemic lupus erythematosus
Journal of Biomedical Science
Volume 10, Issue 5 , pp 544-551
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- Kluwer Academic Publishers
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- Cross-reactivity DNA
- Author Affiliations
- 1. Department of Medical Technology, College of Medicine, National Cheng Kung University, 701, Tainan, Taiwan (ROC)
- 2. Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC
- 3. Department of Pathology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC
- 4. Department of Internal Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC