Journal of Biomedical Science

, Volume 5, Issue 4, pp 290–296

Establishment and characterization of NS3 protein-specific T-cell clones from a patient with chronic hepatitis C

  • Bor-Luen Chiang
  • Pei-Ming Yang
  • Lih-Hwa Hwang
  • Jo-Man Wang
  • Shing-Fen Kao
  • Chien-Hsiung Pan
  • Wei-Kuang Chi
  • Pei-Jer Chen
  • Ding-Shinn Chen
Original Paper

DOI: 10.1007/BF02255861

Cite this article as:
Chiang, B., Yang, P., Hwang, L. et al. J Biomed Sci (1998) 5: 290. doi:10.1007/BF02255861

Abstract

Our previous study showed dominant proliferative response of peripheral mononuclear cells to hepatitis C virus (HCV) nonstructural (NS-3) (T9, from aa 1188 to 1493) in chronically infected patients. Six T9-specific T-cell clones derived in an HCV patient were established and studied for the antigen specificity and the ability of augmentation of in vitro antibody production. All these cloned T-cell lines responded exclusively to T9 antigen and could help autologous B cells in producing anti-T9 antibody in vitro. Cytokine mRNAs of these T cells was detected by polymerase chain reaction and predominant IL-2 and IFN-γ production was noted. In addition, further elucidation of T-cell antigenic determinant and MHC restriction suggested that these T-cell clones recognized at least two different T-cell antigenic determinants within the NS-3 region in an HLA DQ2-restricted manner. We believe characterization of HCV-specific T-cell responses, especially T-cell epitope mapping and cytokine production pattern, may shed light on further understanding the pathogenic mechanism and designing therapy for HCV infection.

Key words

Hepatitis C virusT-cell clonesCytokines

Copyright information

© National Science Council 1998

Authors and Affiliations

  • Bor-Luen Chiang
    • 1
  • Pei-Ming Yang
    • 2
  • Lih-Hwa Hwang
    • 3
  • Jo-Man Wang
    • 1
  • Shing-Fen Kao
    • 1
  • Chien-Hsiung Pan
    • 1
  • Wei-Kuang Chi
    • 4
  • Pei-Jer Chen
    • 1
  • Ding-Shinn Chen
    • 5
    • 2
    • 3
  1. 1.Graduate Institute of Clinical MedicineNational Taiwan UniversityTaipeiTaiwan ROC
  2. 2.Department of Internal MedicineNational Taiwan UniversityTaipeiTaiwan ROC
  3. 3.Hepatitis Research Center, College of MedicineNational Taiwan UniversityTaipeiTaiwan ROC
  4. 4.Process Development Division, Development Center for BiotechnologyTaipeiTaiwan ROC
  5. 5.Hepatitis Research CenterNational Taiwan University HospitalTaipeiTaiwan/ROC