Journal of Biomedical Science

, Volume 2, Issue 4, pp 384–389

Isolation and characterization ofVibrio vulnificus inhabiting the marine environment of the southwestern area of Taiwan

  • Lien-I Hor
  • Chi-Tai Goo
  • Lei Wan
Original Paper

DOI: 10.1007/BF02255226

Cite this article as:
Hor, LI., Goo, CT. & Wan, L. J Biomed Sci (1995) 2: 384. doi:10.1007/BF02255226

Abstract

Vibrio vulnificus, a marine bacterium, is of concern in Taiwan because it causes wound infections and sepsis with a high mortality rate every year. To examine forV. vulnificus, 13 samples of seawater or oysters were collected from nine sites in Yunlin, Chiayi, and Tainan. Seventy-seven strains ofV. vulnificus were isolated from 11 samples. Among these environmental isolates, 72 (91%) were indole-positive, a characteristic of biotype 1. The remaining five strains although indole-negative, a characteristic previously found exclusively in biotype 2 strains, were all ornithine decarboxylase- and mannitol-positive, which has never been reported for biotype 2 strains. Based on the overall biochemical reactions obtained using a commercial identification system, these indole-negative strains appeared to be more like biotype 1. Fifty-seven ribotypes were identified among these isolates, indicating the great genetic divergence in this species. Of the 30 environmental isolates tested, 17 (56.7%) exhibited virulence comparable to the clinical isolates in the mouse, implying that a high proportion of theV. vulnificus strains in the marine environments might be pathogenic to humans.

Key Words

Vibrio vulnificus Identification Ribotype Virulence in mice 

Copyright information

© National Science Council 1995

Authors and Affiliations

  • Lien-I Hor
    • 1
  • Chi-Tai Goo
    • 1
  • Lei Wan
    • 1
  1. 1.Department of Microbiology and ImmunologyCollege of Medicine National Cheng Kung UniversityTainanTaiwan (ROC)