Isolation and characterization ofVibrio vulnificus inhabiting the marine environment of the southwestern area of Taiwan
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Vibrio vulnificus, a marine bacterium, is of concern in Taiwan because it causes wound infections and sepsis with a high mortality rate every year. To examine forV. vulnificus, 13 samples of seawater or oysters were collected from nine sites in Yunlin, Chiayi, and Tainan. Seventy-seven strains ofV. vulnificus were isolated from 11 samples. Among these environmental isolates, 72 (91%) were indole-positive, a characteristic of biotype 1. The remaining five strains although indole-negative, a characteristic previously found exclusively in biotype 2 strains, were all ornithine decarboxylase- and mannitol-positive, which has never been reported for biotype 2 strains. Based on the overall biochemical reactions obtained using a commercial identification system, these indole-negative strains appeared to be more like biotype 1. Fifty-seven ribotypes were identified among these isolates, indicating the great genetic divergence in this species. Of the 30 environmental isolates tested, 17 (56.7%) exhibited virulence comparable to the clinical isolates in the mouse, implying that a high proportion of theV. vulnificus strains in the marine environments might be pathogenic to humans.
- Blumberg HM, Kiehlbauch JA, Wachsmuth IK. Molecular epidemiology ofYersinia enterocolitica O:3 length polymorphisms of rRNA genes. J Clin Microbiol 29:2368–2374;1991.
- Blumberg HM, Stephens DS, Licitra C, Pigott N, Facklam R, Swaminathan B, Wachsmuth IK. Molecular epidemiology of group B streptococcal infections: use of restriction endonuclease analysis of chromosomal DNA and DNA restriction fragment length polymorphisms of ribosomal RNA genes (ribotyping). J Infect Dis 166:574–579;1992.
- Buchrieser C, Ganger VV, Murphee RL, Tamplin ML, Kasper CW. Genomic typing ofVibrio vulnificus using clamped homogeneous electric fields (CHEF) electrophoresis. Abstr 94th Gen Meet Am Soc Microbiol, abstr P-46, p 377;1994.
- Chuang Y-C, Yuan C-Y, Liu C-Y, Lan C-K, Huang AH-M.Vibrio vulnificus infection in Taiwan: report of 28 cases and review of clinical manifestations and treatment. Clin Infect Dis 15:1–6;1992.
- Farmer III JJ, Hickman-Brenner FW. The generaVibrio andPhotobacterium. In: Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH, eds. The Prokaryotes, ed 2. New York, Springer, III:2978–2983;1992.
- Hayat U, Reddy GP, Bush CA, Johnson JA, Wright AC, Morris Jr JG. Capsular types ofVibrio vulnificus: an analysis of strains from clinical and environmental sources. J Infect Dis 168:758–762;1993.
- Hill WE, Keasler SP, Trucksess MW, Feng P, Kaysner CA, Lampel KA. Polymerase chain reaction identification ofVibrio vulnificus in artificially contaminated oysters. Appl Environ Microbiol 57:707–711;1991.
- Hlady WG, Mullen RC, Hopkins RS.Vibrio vulnificus infections associated with raw oyster consumption-Florida, 1981–1992. Arch Dermatol 129:957–958;1993.
- Irino K, Grimont F, Casin I, Grimont PAD, the Brazilian purpuric fever study group. rRNA gene restriction patterns ofHemophilus influenzae biogroup aegyptius strains associated with Brazilian purpuric fever. J Clin Microbiol 26:1535–1538;1988.
- Klontz KC, Lieb S, Schreiber M, Janowski HT, Balda LM, Gunn RA. Syndromes ofVibrio vulnificus infections. Clinical and epidemiologic features in Florida cases, 1981–1987. Ann Intern Med 109:318–323;1988.
- Maloy SR. Experimental Techniques in Bacterial Genetics. Boston, Jones & Bartlett, 1990.
- Massad G, Oliver JD. New selective and differential medium forVibrio cholerae andVibrio vulnificus. Appl Environ Microbiol 53:2262–2264;1987.
- Park SD, Shon HS, Joh NJ.Vibrio vulnificus septicemia in Korea: clinical and epidemiologic findings in seventy patients. J Am Acad Dermatol 24:397–403;1991.
- Simpson LM, White VK, Zane SF, Oliver JD. Correlation between virulence and colony morphology inVibrio vulnificus. Infect Immun 55:269–272;1987.
- Singer PD, Tall BD, Khambaty FM, Shah DB. Genomic restriction pattern diversity amongVibrio vulnificus from different environments. Abstr 94th Gen Meet Am Soc Microbiol, abstr C-417, p 564;1994.
- Song Y-L, Cheng W, Shen C-H, Sung H-H. Occurrence ofVibrio vulnificus infections in cultured shrimp and eel in Taiwan. Proc ROC-JAPAN Symp Fish Dis 172–179;1990.
- Stull TL, Lipuma JJ, Edlind TD. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis 157:280–286;1988.
- Tison TL, Nishibuchi M, Greenwood JD, Seidler RJ.Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. Appl Environ Microbiol 44:640–646;1982.
- Wright AC, Simpson LM, Oliver JD. Role of iron in the pathogenesis ofVibrio vulnificus infections. Infect Immun 34:503–507;1981.
- Wu J-J, Hor L-I, Shiau S-L. Differentiation ofVibrio vulnificus strains by an arbitrarily primed polymerase chain reaction. Chin J Microbiol Immunol; submitted.
- Yoshida SI, Ogawa M, Mizuguchi Y. Relation of capsular materials and opacity to virulence ofVibrio vulnificus. Infect Immun 47:446–451;1985.
- Isolation and characterization ofVibrio vulnificus inhabiting the marine environment of the southwestern area of Taiwan
Journal of Biomedical Science
Volume 2, Issue 4 , pp 384-389
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- Vibrio vulnificus
- Virulence in mice