Journal of Biomedical Science

, Volume 4, Issue 5, pp 235–243

Application of recombinant rhodostomin in studying cell adhesion

  • Hsin-Hou Chang
  • Chih-Pei Chang
  • Jui-Chin Chang
  • Shing-Zeng Dung
  • Szecheng J. Lo
Meeting Report

DOI: 10.1007/BF02253423

Cite this article as:
Chang, HH., Chang, CP., Chang, JC. et al. J Biomed Sci (1997) 4: 235. doi:10.1007/BF02253423

Abstract

Rhodostomin from venom ofAgkistrodon rhodostoma (also calledCalloselasma rhodostoma) contains 68 amino acid residues including 6 pairs of disulfide bonds and an arginine-glycine-aspartic acid (RGD) sequence at positions 49–51. It has been known as one of the strongest antagonists to platelet aggregation among the family termed disintegrin. In this review paper, in addition to introducing the characteristics of disintegrin and its related molecules, the advantages of using recombinant DNA technology to produce rhodostomin are described. The recombinant rhodostomin has been demonstrated to facilitate cell adhesion via interaction between the RGD motif of rhodostomin and integrins on the cell surface. This property allowed us to use the recombinant rhodostomin as an extracellular matrix to study cell adhesion and to distinguish attachment efficiency between two melanoma cell lines B16-F1 and B16-F10, the former is a low metastasis cell while the latter is a high metastasis cell. Furthermore, by using the recombinant rhodostomin as a substrate, osteoprogenitor-like cells are able to be selected and enriched within 3 days from rat bone marrow which contains a heterogeneous cell population. Finally, we show that the recombinant rhodostomin can be immobilized on beads and which serve as an affinity column to dissect cell-surface protein(s) binding to the RGD motif of rhodostomin.

Key Words

KistrinDisintegrinIntegrinExtracellular matrixMetastasisBone marrow stromal cell

Copyright information

© National Science Council 1997

Authors and Affiliations

  • Hsin-Hou Chang
    • 2
  • Chih-Pei Chang
    • 2
  • Jui-Chin Chang
    • 2
  • Shing-Zeng Dung
    • 1
  • Szecheng J. Lo
    • 2
  1. 1.Institute of Clinical Dentistry, School of MedicineNational Yang-Ming UniversityTaipeiTaiwan
  2. 2.Institute of Microbiology and Immunology School of Life ScienceNational Yang-Ming UniversityTaipeiTaiwan 11221 (ROC)