Longitudinal body wall muscles are electrically coupled across the segmental boundary in the third instar larva ofDrosophila melanogaster
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Longitudinal body wall muscles in the third instar larva of the fruitfly,Drosophila melanogaster, were systematically examined for electrical and dye coupling. These muscle cells were found to be electrically coupled but rarely dye-coupled across the segmental boundary. The inter-segmental coupling coefficients between muscle #6s and muscle #7s across the segmental boundary were 0.33 ± 0.09 (mean ± S.D.,n = 12) and 0.43 ± 0.09 (n = 5), respectively, which are much larger than values previously reported inDrosophila but similar to those reported in the blowfly and hawkmoth. By contrast, the intra-segmental coupling coefficient between muscles #6 and #7 was smaller, 0.16 ± 0.08 (n = 28). Other muscle cells which had apparent physical contacts with these longitudinal muscles were examined but were not electrically coupled to them. Nerve-evoked as well as miniature excitatory junctional potentials were found also electrotonically spread across the segmental boundary. The inter-segmental coupling between muscle #6s was not blocked by the gap junction inhibitors halothane or 1-octanol. Functional significance of this electrical coupling is apparently in coordination of larval body movements.
- Bate, M. (1990) The embryonic development of larval muscles inDrosophila.Development,110, 791–804.
- Bennett, M. V. L., Barrio, L. C., Bargiello, T. A., Spray, D. C., Hertzberg, E. and Saez, J. C. (1991) Gap junctions: New tools, new answers, new questions.Neuron,6, 305–320.
- Bodmer, R., Verselis, V., Levitan, I. B. and Spray, D. C. (1988) Electrotonic synapses betweenAplysia neurons insitu and in culture: Aspects of regulation and measurements of permeability.J. Neurosci.,8, 1656–1670.
- Broadie, K. S. and Bate, M. (1993a) Development of embryonic neuromuscular synapse ofDrosophila mehnogaster.J. Neurosci.,13, 144–166.
- Broadie, K. S. and Bate, M. (1993b) Innervation directs receptor synthesis and localization inDrosophila embryo synaptogenesis.Nature,361, 350–353.
- Burt, J. M. and Spray, D. C. (1989) Volatile anesthetics block intercellular communication between neonatal rat myocardial cells.Circ. Res.,65, 829–837.
- DiAntonio, A., Parfitt, K. D. and Schwarz, T. L. (1993) Synaptic transmission persists in synaptotagmin mutants ofDrosophila.Cell,73, 1281–1290.
- Duncan, G., Stewart, S., Prescott, A. R. and Warn, R. M. (1988) Membrane and junctional properties of the isolated frog lens epithelium.J. Membr. Biol.,102, 195–204.
- Gho, M. (1994) Voltage-clamp analysis of gap junctions between embryonic muscles inDrosophila.J. Physiol. (Lond.),481, 371–383.
- Hardie, J. (1978) Electrotonic coupling between supercontracting body-wall muscle fibres and their attachment morphology in the blowfly larva.J. Insect Physiol.,24, 647–655.
- Haugland, F. N. and Wu, C.-F. (1990) A voltage-clamp analysis of gene-dosage effects of theshaker locus on larval muscle potassium currents inDrosophila.J. Neurosci.,10, 1357–1371.
- Jan, L. Y. and Jan, Y. N. (1976) Properties of the larval neuromuscular junction inDrosophila melanogaster.J Physiol. (Lond.),262, 189–214.
- Johansen, J., Halpern, M. E. and Keshishian, H. (1989) Axonal guidance and the development of muscle fiber-specific innervation inDrosophila embryos.J. Neurosci.,9, 4318–4332.
- Johnston, M. F., Simon, S. A. and Ramon, F. (1980) Interaction of anesthetics with electrical synapses.Nature,286, 498–500.
- Kidokoro, Y. and Nishikawa, K. (1994) Miniature endplate currents at the newly formed neuromuscular junction inDrosophila embryos and larvae.Neurosci. Res.,19, 143–154.
- Moreno, A. P., Eghbali, B. and Spray, D. C. (1991) Connexin 32 gap junction channels in stably transfected cells: Unitary conductance.Biophys. J.,60, 1254–1266.
- Nishikawa, K. and Kidokoro, Y. (1995) Junctional and extrajunctional glutamate receptor channels inDrosophila embryos and larvae.J. Neurosci. 15, 7905–7915.
- Rup, D. M., Veenstra, R. D., Wang, H.-Z., Brink, P. R. and Beyer, E. C. (1993) Chick connexin 56, a novel lens gap junction protein. Molecular cloning and functional expression.J. Biol. Chem.,268, 706–712.
- Schwartz, L. (1981) Transegmental electrical coupling between adjacent intersegmental muscles in the tobacco hawkmoth (Manduca sexta).J. Insect Physiol.,27, 727–734.
- Spray, D. C., Moreno, A. P., Kessler, J. A. and Dermietzel, R. (1991) Characterization of gap junctions between cultured leptomeningeal cells.Brain Res.,568, 1–14.
- Stewart, B. A., Atwood, H. L., Renger, J. J., Wang, J. and Wu, C.-F. (1994) Improved stability ofDrosophila larval neuromuscular preparations in haemolymph-like physiological solutions.J. Comp. Physiol. A,175, 179–191.
- Wang, J., Reger, J. J., Griffith, L. C., Greenspan, R. J. and Wu, C.-F. (1994) Concomitant alterations of physiological and developmental plasticity inDrosophila CaM kinase II-inhibited synapses.Neuron,13, 1373–1384.
- Zhong, Y. and Wu, C.-F. (1991a) Altered synaptic plasticity inDrosophila memory mutants with a defective cyclic AMP cascade.Science,251, 198–201.
- Zhong, Y. and Wu, C.-F. (1991b). Alteration of four identified K+ currents inDrosophila muscle by mutations ineag.Science,252, 1562–1564.
- Zhong, Y., Budnik, V. and Wu, C.-F. (1992) Synaptic plasticity inDrosophila memory and hyperexcitable mutants: role of cAMP cascade.J. Neurosci.,12, 644–651.
- Longitudinal body wall muscles are electrically coupled across the segmental boundary in the third instar larva ofDrosophila melanogaster
Volume 1, Issue 4 , pp 315-322
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Drosophila melanogaster
- 3rd instar larvae
- larval muscles
- electrical coupling
- synaptic transmission
- Industry Sectors