Current Microbiology

, Volume 21, Issue 6, pp 377–379

Characterization ofCampylobacter jejuni lipopolysaccharide

  • Robert S. Conrad
  • Chris Galanos
Article

DOI: 10.1007/BF02199441

Cite this article as:
Conrad, R.S. & Galanos, C. Current Microbiology (1990) 21: 377. doi:10.1007/BF02199441

Abstract

Lipopolysaccharides were isolated from dehydratedCampylobacter jejuni by combination of the phenol-chloroform-petroleum ether and phenol-water extraction techniques. Biochemical characterizations of lipopolysaccharide were performed on the two fractions of highest purity. Neutral sugar analyses detected galactose, glucose, trace amounts of mannose, and an unidentified deoxy-hexose. The primary amino sugars were galactosamine, glucosamine, and glucosamine-phosphate. Chemical analyses of other lipopolysaccharide components included phosphate, 3-deoxy-d-manno-octulosonic acid (KDO), and fatty acids. The predominant fatty acids were 3-hydroxytetradecanoic and hexadecanoic acids with lesser amounts of tetradecanoic acid. 3-Hydroxytetradecanoic acids were bound to lipid A by both amide and ester linkages.

Copyright information

© Springer-Verlag New York Inc 1990

Authors and Affiliations

  • Robert S. Conrad
    • 1
  • Chris Galanos
    • 2
  1. 1.Department of Biochemistry and Microbiology, College of Osteopathic MedicineOklahoma State UniversityTulsaUSA
  2. 2.Max-Planck-Institute for ImmunobiologyFreiburgGermany