Original Paper

Molecular and General Genetics MGG

, Volume 248, Issue 4, pp 491-498

First online:

A fission yeast gene mapping close tosuc1 encodes a protein containing two bromodomains

  • Stephen J. AvesAffiliated withDepartment of Biological Sciences, University of Exeter, Washington Singer Laboratories
  • , John HindleyAffiliated withDepartment of Biochemistry, University of Bristol
  • , Geraldine A. PhearAffiliated withDepartment of Biochemistry, University of Bristol
  • , Nicholas TongueAffiliated withDepartment of Biological Sciences, University of Exeter, Washington Singer Laboratories

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Abstract

A novel gene,brd1, has been cloned from the fission yeastSchizosaccharomyces pombe. The predictedbrd1 product contains two copies of an imperfect repeat of 96 amino acid residues in its N-terminal half. These each include a region with high homology to the bromodomains found in transcriptional activator proteins from a diversity of eukaryotes. An in vivo deletion of the completebrd1 open reading frame is not lethal but cells exhibit thermosensitivity, with reductions in both cell growth and stationary phase survival at 36°C.brd1 maps adjacent to the genesuc1, but is expressed separately to give a low abundance 2.1 kb mRNA.

Key words

Schizosaccharomyces pombe Bromodomain Transcriptional activation Coactivator Stationary phase