European Journal of Clinical Microbiology and Infectious Diseases

, Volume 14, Issue 1, pp 1–5

Polymerase chain reaction for detection ofBorrelia burgdorferi DNA in skin lesions of early and late Lyme borreliosis

  • L. V. von Stedingk
  • I. Olsson
  • H. S. Hanson
  • E. Åsbrink
  • A. Hovmark
Article

DOI: 10.1007/BF02112610

Cite this article as:
von Stedingk, L.V., Olsson, I., Hanson, H.S. et al. Eur. J. Clin. Microbiol. Infect. Dis. (1995) 14: 1. doi:10.1007/BF02112610

Abstract

The aim of this study was to evaluate the polymerase chain reaction (PCR) as a diagnostic tool for Lyme borreliosis on large numbers of samples from clinically well-defined cases of early and late cutaneous borreliosis. Skin biopsy specimens from patients with erythema migrans and acrodermatitis chronica atrophicans were analysed blindly together with an equal number of control biopsies. Using two different dilutions of each DNA specimen increased the number of total positives detected. All of the 76 control biopsies were PCR negative. Biopsy specimens from 18 of 26 (69 %) erythema migrans lesions and from 22 of 36 (61 %) acrodermatitis chronica atrophicans lesions were PCR positive. Fourteen post-therapy biopsies from patients with acrodermatitis chronica atrophicans were all negative, supporting the opinion that antibiotic therapy is successful in this chronic manifestation of Lyme borreliosis.

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1995

Authors and Affiliations

  • L. V. von Stedingk
    • 1
  • I. Olsson
    • 2
  • H. S. Hanson
    • 1
  • E. Åsbrink
    • 2
  • A. Hovmark
    • 2
  1. 1.Department of Clinical MicrobiologyKarolinska HospitalStockholmSweden
  2. 2.Department of DermatologyKarolinska Institute at SödersjukhusetStockholmSweden