European Journal of Clinical Microbiology and Infectious Diseases

, Volume 13, Issue 10, pp 827–829

Evaluation of a commercial rRNA target amplification assay for detection ofMycobacterium tuberculosis complex in respiratory specimens

Authors

  • S. Ehlers
    • Institut für Medizinische Mikrobiologie und InfektionsimmunologieFreie Universität Berlin
  • M. Pirmann
    • Institut für Medizinische Mikrobiologie und InfektionsimmunologieFreie Universität Berlin
  • W. Zaki
    • Institut für Medizinische Mikrobiologie und InfektionsimmunologieFreie Universität Berlin
  • H. Hahn
    • Institut für Medizinische Mikrobiologie und InfektionsimmunologieFreie Universität Berlin
Note

DOI: 10.1007/BF02111344

Cite this article as:
Ehlers, S., Pirmann, M., Zaki, W. et al. Eur. J. Clin. Microbiol. Infect. Dis. (1994) 13: 827. doi:10.1007/BF02111344

Abstract

Seventy-one respiratory samples were analyzed in a new commercially available target rRNA amplification assay aimed at detectingMycobacterium tuberculosis complex directly in patient specimens. The assay (Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test) correctly identified 26 of 27 samples positive forMycobacterium tuberculosis and 43 of 44 samples negative forMycobacterium tuberculosis in culture. When appropriate controls are performed, the assay also performs well with other specimens, such as tissue biopsies, lymph node or bone marrow aspirates and pleural and ascitic exudates.

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© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994