Journal of Molecular Evolution

, Volume 30, Issue 2, pp 182–188

Conservatism of sites of tRNA loci among the linkage groups of severalDrosophila species

  • John Tonzetich
  • Shizu Hayashi
  • T. A. Grigliatti

DOI: 10.1007/BF02099944

Cite this article as:
Tonzetich, J., Hayashi, S. & Grigliatti, T.A. J Mol Evol (1990) 30: 182. doi:10.1007/BF02099944


The sites of seven tRNA genes (Arg-2, Lys-2, Ser-2b, Ser-7, Thr-3, Thr-4, Val-3b) were studied by in situ hybridization.125I-labeled tRNA probes fromDrosophila melanogaster were hybridized to spreads of polytene chromosomes prepared from fourDrosophila species representing different evolutionary lineages (D. melanogaster, Drosophila hydei, Drosophila pseudoobscura, andDrosophila virilis). Most tRNA loci occurred on homologous chromosomal elements of all four species. In some cases the number of hybridization sites within an element varied and sites on nonhomologous elements were found. It was observed that both tRNA2Arg and tRNA2Lys hybridized to the same site on homologous elements in several species. These data suggest a limited amount of exchange among different linkage groups during the evolution ofDrosophila species.

Key words

Drosophila species tRNA In situ hybridization Polytene chromosomes Chromosomal evolution 

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • John Tonzetich
    • 1
  • Shizu Hayashi
    • 2
  • T. A. Grigliatti
    • 3
  1. 1.Department of BiologyBucknell UniversityLewisburgUSA
  2. 2.Department of BiochemistryUniversity of British ColumbiaVancouverCanada
  3. 3.Department of ZoologyUniversity of British ColumbiaVancouverCanada