Digestive Diseases and Sciences

, Volume 41, Issue 2, pp 421–426

NoMycobacterium paratuberculosis found in crohn's disease using the polymerase chain reaction

  • Jean-Marc Dumonceau
  • André van Gossum
  • Michael Adler
  • Pierre-Alain Fonteyne
  • Jean-Paul Van Vooren
  • Jacques Devière
  • Françoise Portaels
Intestinal Disorders, Inflammatory Bowel Disease, Immunology, And Microbiology

DOI: 10.1007/BF02093838

Cite this article as:
Dumonceau, J., van Gossum, A., Adler, M. et al. Digest Dis Sci (1996) 41: 421. doi:10.1007/BF02093838

Abstract

M. paratuberculosis has been considered as a putative causative factor of Crohn's disease. However, its detection in diseased tissue samples using the polymerase chain reaction yielded conflicting results. We validated this technique for the detection of mycobacteria (any species) andM. paratuberculosis before applying it to 72 intestinal biopsies from patients with Crohn's disease (N=36), ulcerative colitis (N=13), and control subjects (N=23). Possible polymerase chain reaction inhibitors were detected by spiking template DNA with the equivalent of twoM. paratuberculosis genomes. Mycobacteria were found in 17/36 (47%), 6/13 (46%), and 13/23 (57%) tissue samples of Crohn's disease, ulcerative colitis, and controls, respectively. NoM. paratuberculosis were detected in any sample. It is concluded that mycobacteria are present with a similar frequency in the intestinal tissues or luminal inclusions of patients with inflammatory bowel disease and of those unaffected by the disease. Our data do not support a role forM. paratuberculosis in Crohn's disease.

Key words

Crohn's diseaseinfectionmycobacteriaM. paratuberculosispolymerase chain reaction

Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Jean-Marc Dumonceau
    • 1
    • 2
  • André van Gossum
    • 1
    • 2
  • Michael Adler
    • 1
    • 2
  • Pierre-Alain Fonteyne
    • 1
    • 2
  • Jean-Paul Van Vooren
    • 1
    • 2
  • Jacques Devière
    • 1
    • 2
  • Françoise Portaels
    • 1
    • 2
  1. 1.From the Department of Gastroenterology, Hôpital ErasmeUniversité Libre de BruxellesBrussels
  2. 2.Institute of Tropical MedicineAntwerpBelgium
  3. 3.Hôpital Erasme, Université Libre de BruxellesBrusselsBelgium