, Volume 41, Issue 10, pp 2078-2086

Increased oxidative stress and decreased antioxidant defenses in mucosa of inflammatory bowel disease

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Abstract

Inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation whose cellular components are capable of oxidative respiratory bursts that may result in tissue injury. Mucosal biopsies were analyzed for protein carbonyl content (POPs), DNA oxidation products [8-hydroxy-2′-deoxyguanosine (8-OHdG)], reactive oxygen intermediates (ROIs), trace metals (copper, zinc, and iron) and superoxide dismutase (Cu-Zn SOD). In Crohn's disease biopsies, there was an increase in ROIs, POPs, 8-OHdG, and iron, while decreased copper and Cu-Zn SOD activity were found in inflamed tissues compared to controls. For ulcerative colitis, there was an increase in ROIs, POPs, and iron in inflamed tissue compared to controls, while decreased zinc and copper were observed. An imbalance in the formation of reactive oxygen species and antioxidant micronutrients may be important in the pathogenesis and/or perpetuation of the tissue injury in IBD and may provide a rationale for therapeutic modulation with antioxidants.

This work was supported in part by the Joe and Emily Lowe Foundation, Reach Out for Youth with Ileitis and Colitis, and a gift from Ruth and Leonard Litwin. Dr. Mullin is the recipient of a Career Development Award from the Crohn's and Colitis Foundation of America and an Investigator's Award from the American College of Gastroenterology.