Current Microbiology

, Volume 20, Issue 2, pp 111–114

Structural requirements for the induction and inhibition of 2,3-dihydroxybenzoic acid decarboxylase ofAspergillus niger

  • Ajith V. Kamath
  • C. S. Vaidyanathan

DOI: 10.1007/BF02092882

Cite this article as:
Kamath, A.V. & Vaidyanathan, C.S. Current Microbiology (1990) 20: 111. doi:10.1007/BF02092882


2,3-Dihydroxybenzoic acid decarboxylase inAspergillus niger was induced by many substrate analogs including salicylate and gentisate. Catechol, which is the product, induced the enzyme tenfold. The purified enzyme was competitively inhibited by manyortho substituted benzoic acids. The Ki values for salicylate,o-fluoro ando-chloro benzoic acids were 0.12 mM, 0.12 mM, and 0.13 mM respectively; these values were lower than the Km value for the substrate. As the size of the group in theortho position increased, as in the case of bromo- and iodo-derivatives, there was an increase in their Ki values. The C-2 hydroxyl group was essential both for the induction and for interaction with the enzyme. The C-3 hydroxyl group was not necessary for induction or inhibition, but it might be essential for the catalysis.

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Ajith V. Kamath
    • 1
  • C. S. Vaidyanathan
    • 1
  1. 1.Department of BiochemistryIndian Institute of ScienceBangaloreIndia
  2. 2.Department of MicrobiologyThe University of Texas at AustinAustinUSA