Digestive Diseases and Sciences

, Volume 39, Issue 5, pp 946–956

Intestinal microflora stimulates myoelectric activity of rat small intestine by promoting cyclic initiation and aboral propagation of migrating myoelectric complex

  • Einar Husebye
  • Per M. Hellström
  • Tore Midtvedt
Article

DOI: 10.1007/BF02087542

Cite this article as:
Husebye, E., Hellström, P.M. & Midtvedt, T. Digest Dis Sci (1994) 39: 946. doi:10.1007/BF02087542

Abstract

Microbial modulation of myoelectric activity in small intestine was studied. Germ-free male Sprague-Dawley rats were equipped with bipolar electrodes from the duodenojejunal junction to the midpoint of small intestine. Prior to and one week after introduction of conventional intestinal microflora, 32±5% and 61±5% (mean±se), respectively, of activity fronts of the migrating myoelectric complex reached the midpoint (P<0.05), and the interval between activity fronts in proximal jejunum was reduced from 31.2±2.0 min to 17.5±0.8 min, respectively (P<0.01). The pattern of propagation was more regular after conventionalization. Slow-wave frequency in proximal jejunum was 38.5±1.2/min in germ-free rats and 43.0±0.8/min in conventional rats (P<0.01), but introduction of microflora failed to increase the frequency in germ-free rats. The frequency of spike potentials succeeding jejunal infusion of 5 ml of 12.5% glucose remained unchanged after conventionalization. Statistical analyses showed that the interval between activity fronts varied mainly within rats, whereas the propagation velocity showed statistically significant variability between rats (P<0.01), regardless of intestinal microflora. Luminal control by the resident microflora is important for physiological cycling and aboral propagation of the migrating myoelectric complex, but seems to be of no major consequence for postprandial myoelectric response.

Key words

gastrointestinal motility germ-free intestinal motility microbiology microflora migrating myoelectric complex myoelectric activity rat small intestine transit 

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Einar Husebye
    • 1
    • 2
  • Per M. Hellström
    • 1
    • 2
  • Tore Midtvedt
    • 1
    • 2
  1. 1.From the Department of Medical Microbial Ecology, Karolinska InstituteKarolinska HospitalStockholmSweden
  2. 2.Department of MedicineKarolinska HospitalStockholmSweden
  3. 3.Medical DepartmentUllevål University HospitalOsloNorway