A standard procedure for cultivating human diploid fibroblastlike cells to study cellular aging
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Human diploid cultures are widely used for the study of aging. We have developed quantitative cell culture procedures that include optimization of culture conditions and determination of replicative age by direct cell count. Using these procedures we are able to determine reproducibly the stage in the replicative life history of diploid cell cultures.
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- A standard procedure for cultivating human diploid fibroblastlike cells to study cellular aging
Journal of tissue culture methods
Volume 6, Issue 3-4 , pp 117-121
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- Kluwer Academic Publishers
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- diploid cells
- population doubling level
- life span
- cell culture