Experientia

, Volume 47, Issue 1, pp 22–31

The human leukemia cell line, THP-1: A multifacetted model for the study of monocyte-macrophage differentiation

  • J. Auwerx
Reviews

DOI: 10.1007/BF02041244

Cite this article as:
Auwerx, J. Experientia (1991) 47: 22. doi:10.1007/BF02041244

Summary

THP-1 is a human monocytic leukemia cell line. After treatment with phorbol esters, THP-1 cells differentiate into macrophage-like cells which mimic native monocyte-derived macrophages in several respects. Compared to other human myeloid cell lines, such as HL-60, U937, KG-1, or HEL cell lines, differentiated THP-1 cells behave more like native monocyte-derived macrophages. Because of these characteristics, the THP-1 cell line provides a valuable model for studying the mechanisms involved in macrophage differentiation, and for exploring the regulation of macrophage-specific genes as they relate to physiological functions displayed by these cells.

Key words

Atherosclerosiscellular differentiationgene expressionfoam cellslipoproteinsphorbol esterstranscription factors

Abbreviations

apo

apolipoprotein

CFU-GM

colony forming unit for the granulocyte-macrophage

CSF

colony stimulating factor

FcR

IgG Fc receptor

HMG-CoA reductase

3-hydroxy 3-methylglutaryl Coenzyme A reductase

γ-IFN

interferon

IL

interleukin

LDL

low density lipoprotein

mAb

monoclonal antibody

PKC

protein kinase C

PMA

phorbol 12-myristate 13-acetate

TNF

tumor necrosis factor-α

Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • J. Auwerx
    • 1
  1. 1.Department of Developmental BiologyLaboratory of Experimental Medicine and EndocrinologyLeuvenBelgium