Archives of Toxicology

, Volume 66, Issue 6, pp 435–442

Urinary creatine profiles after administration of cell-specific testicular toxicants to the rat

  • Nigel P. Moore
  • Dianne M. Creasy
  • Tim J. B. Gray
  • John A. Timbrell
Original Investigations

DOI: 10.1007/BF02035135

Cite this article as:
Moore, N.P., Creasy, D.M., Gray, T.J.B. et al. Arch Toxicol (1992) 66: 435. doi:10.1007/BF02035135


Cell-specific testicular toxicants have been used to examine the distribution of creatine within the rat testis, and to examine the potential use of creatinuria as a non-invasive indicator of testicular damage. Groups of rats were administered single doses of various toxicants: a germ cell toxicant (methoxyacetic acid, MAA), one of two Sertoli cell toxicants (di-n-pentyl phthalate, DPP or 1,3-dinitrobenzene, 1,3-DNB), or a Leydig cell toxicant (ethane-1,2-dimethane sulphonate, EDS). Urinary creatine and creatinine levels were monitored in 24 h periods over the following 48 h, after which time the testes were removed, weighed and, after processing, sections were examined by light microscopy. All four treatments resulted in reductions in relative testis weight (RTW) and produced morphological changes similar to those which have been previously reported. In addition, MAA, DPP and 1,3-DNB all caused significant elevations in urinary creatine excretion and the urinary creatine: creatinine ratio (Cr/Crn) within 24 h. EDS had no such effect. We conclude that creatine is associated with the cells of the seminiferous epithelium, and that elevated urinary excretion of creatine may serve as a non-invasive marker for damage to these cells in vivo.

Key words

Methoxyacetic acidDi-n-pentyl phthalate1,3-DinitrobenzeneEthane-1,2-dimethane sulphonateTestisCreatineCreatinuria



urinary molar creatine:creatinine ratio




di-n-pentyl phthalate


ethane-1,2-dimethane sulphonate


methoxyacetic acid




relative testis weight

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Nigel P. Moore
    • 1
  • Dianne M. Creasy
    • 2
  • Tim J. B. Gray
    • 2
  • John A. Timbrell
    • 1
  1. 1.Toxicology Unit, The School of PharmacyUniversity of LondonLondonUK
  2. 2.British Industrial Biological Research AssociationSurreyUK
  3. 3.BP Nutrition Consumer Products DivisionLondonUK
  4. 4.Pathology DepartmentShell Research Ltd, Sittingbourne Research CentreSittingbourne, KentUK
  5. 5.Sterling Winthrop Research CentreAlnwick, NorthumberlandUK