Journal of Plant Growth Regulation

, Volume 7, Issue 4, pp 203–211

Jasmonic acid-dependent increases in the level of specific polypeptides in soybean suspension cultures and seedlings

Authors

  • James Michael Anderson
    • United States Department of Agriculture, Agricultural Research Service, Departments of Crop Science and BotanyNorth Carolina State University
Article

DOI: 10.1007/BF02025263

Cite this article as:
Anderson, J.M. J Plant Growth Regul (1988) 7: 203. doi:10.1007/BF02025263

Abstract

Adding 2–30 μM jasmonic acid (JA) to photomixotrophic suspension cultures of soybean increased the level of several soluble polypeptides isolated by SDS-PAGE. The major polypeptides affected by JA treatment were at Mr 31,200 (p31) and Mr 39,000. Spraying leaves of soybean seedlings with 10–50 μM JA also increased the level of several soluble polypeptides including p31. The use of Con A affinity chromatography demonstrated that p31 was a glycoprotein and that JA increased the level of three other glycoproteins at Mr 22,000, 33,000, and 52,000. The JA treatment did not alter the growth or morphology of the seedlings. JA at 2–30 μM did not significantly inhibit the growth of the cultured cells and did not significantly alter the chlorophyll concentration. However, JA at concentrations above 30 μM inhibited growth and chlorophyll levels in cultured cells. The suspension cultured cells could provide a reliable bioassay for jasmonic acid.

Copyright information

© Springer-Verlag New York Inc. 1988