Agents and Actions

, Volume 28, Issue 1, pp 9–15

The effect of azelastine and some other antiasthmatic and antiallergic drugs on calmodulin and protein kinase C

  • E. MiddletonJr.
  • P. Ferriola
  • G. Drzewiecki
  • R. Duane Sofia
Allergy, Histamine and Kinins

DOI: 10.1007/BF02022974

Cite this article as:
Middleton, E., Ferriola, P., Drzewiecki, G. et al. Agents and Actions (1989) 28: 9. doi:10.1007/BF02022974

Abstract

The antiallergic and antiasthmatic drug, azelastine, interacts strongly with calmodulin (but not bovine serum albumin) as determined by an indirect assay; it also moderately inhibited the Ca2+-calmodulindependent enzyme bovine brain phosphodiesterase. Ketotifen was less active than azelastine in both assays of calmodulin reactivity and both drugs were less active than the recognized calmodulin inhibitor, W-7. Neither azelastine nor ketotifen had any inhibitory effect on the Ca2+- and phospholipid-dependent protein kinase C. A number of other commonly employed antiallergic and antiasthmatic drugs were essentially inactive in the calmodulin assays and had no or marginal inhibitory effect on protein kinase C.

Copyright information

© Birkhäuser Verlag 1989

Authors and Affiliations

  • E. MiddletonJr.
    • 1
  • P. Ferriola
    • 1
  • G. Drzewiecki
    • 1
  • R. Duane Sofia
    • 2
  1. 1.Department of Medicine State University of New York at BuffaloBuffaloUSA
  2. 2.Wallace LaboratoriesCranburyUSA