European Journal of Clinical Microbiology

, Volume 3, Issue 3, pp 249–252

Relative proportions ofHaemophilus species in the throat of healthy children and adults

  • D. Kuklinska
  • M. Kilian
Fastidious Gram-Negative, Facultatively Anaerobic Rods: Identification and Clinical Significance

DOI: 10.1007/BF02014895

Cite this article as:
Kuklinska, D. & Kilian, M. Eur. J, Clin. Microbiol. (1984) 3: 249. doi:10.1007/BF02014895


To determine normal proportions of pharyngealHaemophilus species, qualitative and quantitative mapping of the species in the pharynx of ten healthy children and ten healthy adults was carried out using a selective and a non-selective medium.Haemophilus organisms were present in all samples, comprising approximately 10 % of the total cultivable flora (range 0.6–36.9 %).Haemophilus parainfluenzae was a member of the normal flora throughout life, constituting 74% of pharyngealHaemophilus organisms.Haemophilus segnis andHaemophilus paraphrophilus occurred more frequently in samples from adults, whereasHaemophilus haemolyticus was present in only one sample. Non-encapsulatedHaemophilus influenzae strains, usually of multiple biotypes, were present in 80% of the children but accounted for a mean of only 1.8 % of the total flora. Their number decreased with increasing age; 40 % of the adults harboredHaemophilus influenzae but only of a single biotype which constituted a minor proportion of the total flora (mean 0.15%). These findings suggest that host mechanisms can influence changes in the proportions ofHaemophilus influenzae strains colonizing the host.

Copyright information

© Vieweg Publishing 1984

Authors and Affiliations

  • D. Kuklinska
    • 1
  • M. Kilian
    • 2
  1. 1.Department of BacteriologyState Institute of HygieneWarsawPoland
  2. 2.Department of Oral BiologyRoyal Dental CollegeAarhus CDenmark