European Journal of Clinical Microbiology

, Volume 5, Issue 2, pp 148–151

Cross-reactivity of monoclonal antibodies against lipopolysaccharides of gram-negative bacteria

  • J. de Jongh-Leuvenink
  • A. S. Bouter
  • J. H. Marcelis
  • J. Schellekens
  • J. Verhoef
Articles

DOI: 10.1007/BF02013970

Cite this article as:
de Jongh-Leuvenink, J., Bouter, A.S., Marcelis, J.H. et al. Eur. J, Clin. Microbiol. (1986) 5: 148. doi:10.1007/BF02013970

Abstract

Monoclonal antibodies were produced againstEscherichia coli O111, Escherichia coli J5, and the rough (R) mutant ofSalmonella typhimurium M206, and tested by enzyme-linked immunosorbent assay against lipopolysaccharides of several gram-negative strains. The monoclonal antibodies were also identified with an immunoblotting assay.Anti-Escherichia coli O111 monoclonal antibodies reacted only with homologous O antigens. Anti-J5 monoclonal antibodies cross-reacted with core lipopolysaccharide, especially with Rc lipopolysaccharide. IgM anti-J5 monoclonal antibodies showed more extensive cross-reactivity than IgG3 monoclonal antibodies. Anti-Re monoclonal antibodies cross-reacted weakly with all rough lipopolysaccharide tested. Thus, the varying specificity of these monoclonal antibodies seems to indicate that the core regions in the lipopolysaccharides of various gramnegative bacteria are not similar.

Copyright information

© Friedr. Vieweg & Sohn 1986

Authors and Affiliations

  • J. de Jongh-Leuvenink
    • 1
  • A. S. Bouter
    • 1
  • J. H. Marcelis
    • 1
  • J. Schellekens
    • 1
  • J. Verhoef
    • 1
  1. 1.Laboratory of MicrobiologyState University of UtrechtGG UtrechtThe Netherlands