The Journal of Membrane Biology

, Volume 123, Issue 1, pp 63–71

Intracellular free zinc and zinc buffering in human red blood cells

  • T. J. B. Simons

DOI: 10.1007/BF01993964

Cite this article as:
Simons, T.J.B. J. Membrain Biol. (1991) 123: 63. doi:10.1007/BF01993964


Zn2+ has been allowed to equilibrate across the red cell membrane using two agents that increase membrane permeability to this ion: the ionophore A23187 and the specific carrier ethylmaltol. Extracellular free Zn2+ was controlled with EGTA (1,2-di(2-aminoethoxy)ethane-NNN′N′tetra-acetic acid)) buffers, except in the case of ethylmaltol, which itself acts as a buffer. Measurement of cellular zinc content at different levels of free Zn2+ facilitated the study of intracellular Zn2+ binding. It was also possible to estimate intracellular free Zn2+ concentration in untreated cells using a “null-point” technique. Intracellular zinc was found to consist of an inexchangeable component of about 129 μmol/1013 cells and an exchangeable component of 6.7±1.5 μmol/1013 cells, with a free concentration of about 2.4×10−11m. The main component of Zn2+ buffering is hemoglobin, with a dissociation constant of about 2×10−8m.

Key Words


Copyright information

© Springer-Verlag New York Inc 1991

Authors and Affiliations

  • T. J. B. Simons
    • 1
  1. 1.Biomedical Sciences DivisionKing's College LondonLondon