Histamine release from the cultured mouse mast cell line PT 18 in response to immunologic and non-immunologic stimuli
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Cultured mouse mast cells that are dependent on spleen-derived factors for their proliferation and maintenance have been shown rapidly to release histamine in response to immunologic stimuli, concanavalin A, and ionophores A23187 and Br-x537A. These cells did not, however, release histamine when treated with compound 48/80 or adenosine 5′-triphosphate. Immunologically mediated histamine release was inhibited by theophylline and quercetin, but not by disodium cromoglycate. The results presented here are discussed in terms of the possible relationship of this cell line to mucosal mast cells.
- E. Razin, R.L. Stevens, F. Akiyama, K. Schmid andK.F. Austen,Culture from mouse bone marrow of a subclass of mast cells possessing a distinct chondroitin sulfate proteoglycan with glycosaminoglycans rich in N-acetylgalactosamine-4,6-disulfate, J. biol. Chem.257, 7229–7236 (1982).
- J.W. Schrader,The in vitro production and cloning of the P-cell, a bone marrow-derived null cell that expresses H-2 and Ia-antigens, has mast cell-like granules, and is regulated by a factor released by activated T cells, J. Immun.126, 452–458 (1981).
- D.H. Pluznik, N.S. Tare, M.M. Zatz andA.L. Goldstein,A mast/basophil cell line dependent on colony stimulating factor, Exp. Hematol.10, 211–218 (1982).
- B. Sredni, M.M. Friedman, C.E., Bland andD.D., Metcalfe,Ultrastructural, biochemical and functional characteristics of histamine-containing cells cloned from mouse bone marrow: tentative identification as mucosal mast cells, J. Immun.131, 915–922 (1983).
- C.E. Bland, K.L. Rosenthal, D.H. Pluznik C. Riedel and andD.D. Metcalfe,Comparison of glycosaminoglycans synthesized by cultured mast cells and granulated lymphocytes, Fedn Proc.42, 444 (1983).
- J.W. Schrader andI. Clark-Lewis,T cell hybridoma-derived regulatory factors I. Production of T cell growth factor following stimulation by concanavalin A, J. Immun.126, 1101–1105 (1981).
- K.E. Barrett, M. Ennis, andF.L. Pearce,Mast cells isolated from guinea pig lung: characterization and studies on histamine secretion, Agents and Actions13, 122–126 (1983).
- G. Atkinson, M. Ennis andF.L., Pearce,The effect of alkaline earth cations on the release of histamine from rat peritoneal, mast cells treated with compound 48/80 and peptide 401 Br. J. Pharmac.65, 395–402 (1979).
- F.L. Pearce, K.E. Barrett andJ.R. White,Histamine secretion from mast cells treated with chlortetracycline (aureomycin): a novel calcium ionophore, Agents and Actions13, 117–122 (1983).
- K.E. Barrett andF.L. Pearce,A comparison of histamine secretion from peritoneal mast cells of the mouse and the rat, Int. Archs Allergy appl. Immun.72, 234–238 (1983).
- P.A. Shore, A. Burkhalter andV.H. Cohn,A method for the fluorimetric assay of histamine in tissues, J. Pharmac. exp. Ther.127, 182–186 (1959).
- F.L. Pearce, A.D. Befus, J. Gauldie andJ. Bienenstock,Mucosal mast cell. II. Effects of anti-allergic compounds on histamine secretion by isolated intestinal mast cells, J. Immun.128, 2481–2486 (1982).
- K.E. Barrett, K.B.P. Leung andF.L. Pearce,Histamine secretion and the effect of anti-allergic compounds on peritoneal mast cells of the rat, mouse and hamster, Br. J. Pharmac.78, 58P (1983).
- W. Kazimierczak andB. Diamant,Mechanisms of histamine release in anaphylactic and anaphylactoid reactions, Prog. Allergy24, 295–365 (1978).
- A. Sydbom andB. Uvnäs,Potentiation of anaphylactic histamine release from isolated rat pleural mast cells by rat serum phospholipids, Acta physiol. scand.97, 222–232 (1976).
- R.P. Siraganian andK.A. Hazard,Mechanisms of mouse mast cell activation and inactivation for IgE-mediated histamine release J. Immun.122, 1719–1725 (1979).
- A.D. Befus, F.L. Pearce, J. Gauldie, P. Horsewood andJ. Bienenstock,Mucosal mast cells I. Isolation and functional characteristics of rat intestinal mast cells, J. Immun.128, 2475–2480 (1982).
- F.L. Pearce andM. Ennis,Isolation and some properties of mast cells from the mesentery of the rat and guinea pig, Agents and Actions10, 124–131 (1980).
- M. Ennis,Histamine release from human pulmonary mast cells, Agents and Actions12, 60–63 (1982).
- K.E. Barrett andF.L. Pearce,A comparative study of histamine secretion from rat peritoneal and pleural mast cells, Agents and Actions12, 186–188 (1982).
- C.M.S. Fewtrell andB.D. Gomperts,Quercetin, a novel inhibitor of Ca 2+ influx and exocytosis in rat peritoneal mast cells, Biochim. biophys. Acta469, 52–60 (1977).
- J. Tas andR.G. Berndsen,Does heparin occur in mucosal mast cells of the rat small intestine? J. Histochem. Cytochem.25 1058–1062 (1977).
- E.J. Ruitenberg andA. Elgersma,Absence of intestinal mast cell response in congenitally athymic mice during Trichinella spiralis infection, Nature264, 258–260 (1976).
- Histamine release from the cultured mouse mast cell line PT 18 in response to immunologic and non-immunologic stimuli
Agents and Actions
Volume 14, Issue 3-4 , pp 488-493
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Allergic Diseases Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, 20205, Bethesda, Maryland, USA
- 2. Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, 20205, Bethesda, Maryland, USA