Archives of Toxicology

, Volume 66, Issue 8, pp 592–597

Mechanisms of cobalt(II) uptake into V79 Chinese hamster cells

  • Ursula Kasten
  • Andrea Hartwig
  • Detmar Beyersmann
Original Investigations

DOI: 10.1007/BF01973391

Cite this article as:
Kasten, U., Hartwig, A. & Beyersmann, D. Arch Toxicol (1992) 66: 592. doi:10.1007/BF01973391

Abstract

V79 Chinese hamster cells were used as a model for the characterization of the Co(II) uptake into mammalian cells as well as the mechanisms involved. Co(II) was taken up in a dose and time dependent manner. The uptake was exponential without saturation in the tested concentration range up to 400 μM. CoCl2. Furthermore, there was a high intracellular cobalt accumulation at elevated extra-cellular Co(II) doses (up to 16 fold at 200 μM). The time course of Co(II) uptake showed a maximum after about 8–12 h with no further change after the longest tested incubation time (24 h). The uptake of Co(II) into V79 cells seems to be mediated by multiple mechanisms: active, energy consuming transport like ion pumps and endocytosis, since the Co(II) uptake was significantly reduced by ouabain (an inhibitor of the Na+/K+ATPase), N-ethyl-maleinimide (an inhibitor of the Ca2+/Mg2+ATPase and the Na+/K+ATPase), chlorpromazine (a calmodulin antagonist and inhibitor of the Ca2+/Mg2+ ATPase) as well as by the endocytosis inhibitor chloroquine. Furthermore, the two agents iodoacetate and potassium cyanide, which produce ATP depletion, resulted in a diminution of the intracellular cobalt concentration. An uptake through anion channels could be excluded, since 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid was not inhibitory

Key words

Cobalt Uptake Mammalian cells Transport mechanisms 

Abbreviations

CFA

colony forming ability

DIDS

4,4′-diisothiocyanostilbene-2,2′-disulphonic acid

EDTA

ethylene diaminotetraacetic acid

MEMα

minimum essential medium, α modified

PBS

phosphate buffered saline

SD

standard deviation

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Ursula Kasten
    • 1
  • Andrea Hartwig
    • 1
  • Detmar Beyersmann
    • 1
  1. 1.Biochemistry Group, Department of Biology and ChemistryUniversity of BremenBremen 33Federal Republic of Germany