Agents and Actions

, Volume 10, Issue 4, pp 323–328

Biochemistry and functional aspects of human glandular kallikreins

  • Reinhard Geiger
  • Edwin Fink
  • Ulrike Stuckstedte
  • Bqruni Förg-Brey
Histamine and Kinins

DOI: 10.1007/BF01971433

Cite this article as:
Geiger, R., Fink, E., Stuckstedte, U. et al. Agents and Actions (1980) 10: 323. doi:10.1007/BF01971433

Abstract

Human urinary kallikrein was purified by gel filtration on Sephacryl S-200 and affinity chromatography on aprotinin-Sepharose, followed by ion exchange chromatography on DEAE-Sepharose. In dodecylsulfate gel electrophoresis two protein bands with molecular weights of 41,000 and 34,000 were separated. The amino acid composition and the carbohydrate content of the kallikrein preparation were determined; isoleucine was identified as the only aminoterminal amino acid. The bimolecular velocity constant for the inhibition by diisopropyl fluorophosphate was determined as 9±2 l mol−1 min−1. The hydrolysis of a number of substrates was investigated and AcPheArgOEt was found to be the most sensitive substrate for human urinary kallikrein. Using this substrate an assay method for kallikrein in human urine was developed.

It was shown by radioimmunoassay that pig pancreatic kallikrein can be absorbed in the rat intestinal tract. Furthermore, in dogs the renal excretion of glandular kallikrein from blood was demonstrated by radioimmunological methods.

Abbreviations

Bz-ArgOEt

Nα-Benzoyl-arginine ethyl ester

Z-TyrONp

Carbobenzoxytyrosine nitrophenyl ester

Ac-Phe-ArgOEt

Acetyl-phenylalanyl-arginine ethyl ester

d-Valleu-ArgOEt

d-valyl-leucyl-arginine ethyl ester

DFP

diisopropyl fluorophosphate

Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • Reinhard Geiger
    • 1
  • Edwin Fink
    • 1
  • Ulrike Stuckstedte
    • 1
  • Bqruni Förg-Brey
    • 1
  1. 1.Department of Clinical Chemistry and Clinical Biochemistry, Surgical ClinicUniversity of MunichMünich 2BRD