Transgenic Research

, Volume 2, Issue 2, pp 115–120

The use of the nonradioactive digoxigenin chemiluminescent technology for plant genomic Southern blot hybridization: A comparison with radioactivity

Authors

  • Gabriele Neuhaus-Url
    • Institute for Plant SciencesSwiss Federal Institute of Technology Zürich
  • Gunther Neuhaus
    • Institute for Plant SciencesSwiss Federal Institute of Technology Zürich
Article

DOI: 10.1007/BF01969385

Cite this article as:
Neuhaus-Url, G. & Neuhaus, G. Transgenic Research (1993) 2: 115. doi:10.1007/BF01969385

Abstract

A nonradioactive labelling and detection method for plant genomic DNA analysis is compared to the radioactive method. The radioisotopes are replaced by a nucleotide, digoxigenin-11-dUTP, and the signal detection is accomplished by the enzymatic reaction of alkaline phosphatase, conjugated to anti-digoxigenin antibodies, with the chemiluminescent substrate AMPPD (3-(2′-spiroadamantane)-4-methoxy-4(3″ phosphorytoxy) phenyl-1, 2-dioxetane). The sensitivity of the radioactive and nonradioactive methods are directly compared using identical Southern blots subjected to the radioactive and nonradioactive detection. The advantages of this nonradioactive method are discussed.

Keywords

chemiluminescencedigoxigeninnonradioactiveplant DNASouthern blot hybridization
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Copyright information

© Chapman & Hall 1993