pBINPLUS: An improved plant transformation vector based on pBIN19
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- van Engelen, F.A., Molthoff, J.W., Conner, A.J. et al. Transgenic Research (1995) 4: 288. doi:10.1007/BF01969123
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We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number inE. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.