Short Communication

Transgenic Research

, Volume 4, Issue 4, pp 288-290

pBINPLUS: An improved plant transformation vector based on pBIN19

  • Fred A. van EngelenAffiliated withDepartment of Molecular Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO)
  • , Jos W. MolthoffAffiliated withDepartment of Molecular Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO)
  • , Anthony J. ConnerAffiliated withDepartment of Molecular Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO)
  • , Jan-Peter NapAffiliated withDepartment of Molecular Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO)
  • , Andy PereiraAffiliated withDepartment of Molecular Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO)
  • , Willem J. StiekemaAffiliated withDepartment of Molecular Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO)

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Abstract

We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number inE. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.

Keywords

transformation vector pBIN19 transgenic plants T-DNA Agrobacterium tumefaciens