European Journal of Clinical Microbiology and Infectious Diseases

, Volume 10, Issue 5, pp 422–427

Diagnosis of Lyme borreliosis by an enzyme immunoassay detecting immunoglobulin G reactive to purifiedBorrelia burgdorferi cell components


  • S. Bergström
    • Department of MicrobiologyUniversity of Umeå
  • A. Sjöstedt
    • Department of BacteriologyUniversity of Umeå
  • L. Dotevall
    • Department of Bacteriology, Sahlgrenska Sjukhuset
    • Department of Infectious Disease, Östra Sjukhuset
  • B. Kaijser
    • Department of Bacteriology, Sahlgrenska Sjukhuset
  • B. Ekstrand-Hammarström
    • Symbicom AB
  • C. Wallberg
    • Symbicom AB
  • G. Skogman
    • Symbicom AB
  • A. G. Barbour
    • Departments of Microbiology and MedicineUniversity of Texas Health Science Center

DOI: 10.1007/BF01968022

Cite this article as:
Bergström, S., Sjöstedt, A., Dotevall, L. et al. Eur. J. Clin. Microbiol. Infect. Dis. (1991) 10: 422. doi:10.1007/BF01968022


An enzyme immunoassay (EIA) developed for the diagnosis of Lyme borreliosis was tested for its specificity and sensitivity in detecting IgG antibodies in patients at various stages of the disease. The EIA is based on a detergent extract ofBorrelia burgdorferi which contains 12 proteins of defined molecular weights fromBorrelia burgdorferi. The assay showed a specificity of 100 % in control sera from 64 healthy individuals, using a cut-off optical density value of 0.13 (\(\bar x \pm 3SD\)). The sensitivity was 100 % using sera from 22 Swedish patients with late stage Lyme borreliosis and 43 % using sera from 30 patients with the initial stage of the disease. The reactivity of the sera against whole cell preparations, the outer surface proteins OspA and OspB, and the flagella ofBorrelia burgdorferi was also tested and compared with the EIA. No cross-reactivity with treponemal antigens was observed when using the EIA.

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1991