Agents and Actions

, Volume 13, Issue 2, pp 122–126

Mast cells isolated from guinea-pig lung: characterization and studies on histamine secretion

  • K. E. Barrett
  • M. Ennis
  • F. L. Pearce
Histamine Release and Mast Cells

DOI: 10.1007/BF01967313

Cite this article as:
Barrett, K.E., Ennis, M. & Pearce, F.L. Agents and Actions (1983) 13: 122. doi:10.1007/BF01967313

Abstract

A method has been developed for the dispersion of guinea-pig lung into its component cells using the proteolytic enzyme collagenase. The procedure typically yielded 5×106 mast cells per g of tissue with a recovery of histamine ofca. 20%. The mast cells comprised 2% of the total nucleated cells, had a histamine content of 1–2 pg per cell and exhibited a low spontaneous release of the amine (ca. 6%). In contrast to the rat peritoneal mast cell, guinea-pig lung mast cells were refractory to the action of compound 48/80, peptide 401 (MCD-peptide), dextran and Concanavalin A. However, the cells released histamine on antigenic challenge following active sensitization and dose-dependent histamine secretion was also produced by the ionophores A23187, ionomycin and Br-X537A. These results further emphasize the functional heterogeneity of mast cells obtained from different species and tissues.

Copyright information

© Birkhäuser Verlag 1983

Authors and Affiliations

  • K. E. Barrett
    • 1
  • M. Ennis
    • 2
  • F. L. Pearce
    • 1
  1. 1.Department of ChemistryUniversity College LondonLondonUK
  2. 2.Department of Clinical PharmacologyRoyal Postgraduate Medical SchoolLondonUK