Research Articles

Experientia

, Volume 47, Issue 5, pp 483-485

First online:

Mitochondrial DNA variation in geographic populations of Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera; Chrysomelidae)

  • A. M. L. Azeredo-EspinAffiliated withInsect Biocontrol Laboratory, ARS-USDA
  • , R. F. W. SchroderAffiliated withInsect Biocontrol Laboratory, ARS-USDA
  • , M. D. HuettelAffiliated withInsect Biocontrol Laboratory, ARS-USDA
  • , W. S. SheppardAffiliated withBee Research Laboratory, ARS-USDA

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Summary

This study demonstrates variability in restriction enzyme cleavage sites of mitochondrial DNA (mtDNA) among four popalations of Colorado potato beetle (CPB). A suite of three enzymes (EcoRI,HpaI,PstI) was sufficient to discriminate among the populations tested. Individuals heteroplasmic for restriction enzyme patterns were found in some populations. Variability in CPB mtDNA should prove useful in efforts to trace the origin and dispersal of the species in North America.

Key words

Mitochondrial DNA RFLP Leptinotarsa decemlineata Colorado potato beetle population genetics