Experientia

, Volume 49, Issue 6, pp 533–538

Interleukin-8 is a Cyclosporin A binding protein

  • H. Bang
  • K. Brune
  • C. Nager
  • U. Feige
Priority Papers

DOI: 10.1007/BF01955157

Cite this article as:
Bang, H., Brune, K., Nager, C. et al. Experientia (1993) 49: 533. doi:10.1007/BF01955157

Abstract

Inflammatory immune reactions occur during transplant rejections and autoimmune diseases. Such reactions are mediated by cytokines, including interleukin-8 (IL-8). Cyclosporin A (CsA) exerts immunosuppressive activities1,2 by binding to immunoregulatory proteins termed cyclophilins3. The anti-inflammatory effects of CsA are still not fully understood. Searching for novel neutrophil-activating proteins, we observed that an antiserum against human recombinant Interleukin-8 (IL-8) cross-reacted with cyclophilins in Western blots. Furthermore, native IL-8 was found to specifically bind CsA, whereas biologically inactive analogs of CsA were not bound by IL-8. Putative binding sites for CsA on IL-8 could be identified on the basis of structural similarities between IL-8 and cyclophilin. However, IL-8 lacks peptidyl-prolyl-isomerase (PPlase) enzyme activity, which is regarded as a characteristic of cyclophilins4,5,6. We conclude that the specific binding of CsA to IL-8 may explain some of the anti-inflammatory effects of CsA. IL-8 may be a novel member of the cyclophilins lacking PPlase activity.

Key words

Cyclosporin AcyclophilinimmunophilinInterleukin-8

Copyright information

© Birkhäuser Verlag Basel 1993

Authors and Affiliations

  • H. Bang
    • 1
  • K. Brune
    • 1
  • C. Nager
    • 2
  • U. Feige
    • 1
  1. 1.Institute of Pharmacology and ToxicologyUniversity of Erlangen-NürnbergErlangen(Germany)
  2. 2.Bio-InformaticsFriedrich-Miescher InstituteBasel(Switzerland)
  3. 3.Pharmaceuticals Research DivisionCIBA Ltd.BaselSwitzerland