Simplification of the ecdysteroid radioimmunoassay by the use of protein A fromStaphylococcus aureus
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- Warren, J.T., Smith, W. & Gilbert, L.I. Experientia (1984) 40: 393. doi:10.1007/BF01952570
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Three methods of separating antibody-bound ligand from free ligand were compared for an ecdysteroid radioimmunoassay. Ecdysteroid antibody concentration and ligand specific radioactivity were adjusted to measure 2 ranges of ecdysone concentrations (0.01–2.0 ng and 0.25–32.0 ng). In comparison with the tradiotional separating agent, ammonium sulfate, neither protein A nor polyethylene glycol altered sensitivity or specificity of the radioimmunoassays. The protein A immunoglobulin precipitation method is quick and simple, making it a preferred alternative protocol for terminating ecdysteroid radioimmunoassays.