Experientia

, Volume 44, Issue 2, pp 169–171

Thymine dimer repair in fibroblasts of patients with dysplastic naevus syndrome (DNS)

  • M. Roth
  • J. M. Boyle
  • Hj. Müller
Short Communications Current Activities in the Department of Research of the University Hospital, Basel

DOI: 10.1007/BF01952205

Cite this article as:
Roth, M., Boyle, J.M. & Müller, H. Experientia (1988) 44: 169. doi:10.1007/BF01952205

Summary

Dysplastic naevus syndrome (DNS) is frequently observed in association with familial melanoma and xeroderma pigmentosum (XP), but the role of UV-light in the development of DNS has not been elucidated. Previous work has shown that UV-induced unscheduled DNA synthesis is associated with the early loss of antigenicity observed in immunoassays using a monoclonal antibody specific for thymine-thymine dimers. We now show that the rate of loss of antigenicity, which reflects the relative amount of bound antibody, observed during the first 60 min following 10 Jm−2 UVC irradiation is significantly reduced (p=0.02) in cultures of fibroblasts from 7 out of 8 DNS patients compared with the results from cells of a group of 30 healthy volunteers. This observation suggests an early event in excision repair is altered in the majority of DNS patients.

Key words

Dysplastic naevus syndrome DNA-repair cancer genes familial malignant melanoma monoclonal antibodies specific for UV-dimers 

Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • M. Roth
    • 1
    • 2
  • J. M. Boyle
    • 3
  • Hj. Müller
    • 1
    • 2
  1. 1.Laboratory of Human Genetics, Dept of Research of the University ClinicsKantonsspitalBasel
  2. 2.Dept of Human GeneticsUniversity Children's HospitalBasel(Switzerland)
  3. 3.Paterson Institute for Cancer ResearchChristie Hospital and Holt Radium InstituteManchester(England)