Experientia

, Volume 42, Issue 7, pp 848–851

Sclerotial and low aflatoxigenic morphological variants from haploid and diploidAspergillus parasiticus

  • J. W. Bennett
  • Phaik-Mooi Leong
  • S. Kruger
  • D. Keyes
Short Communications

DOI: 10.1007/BF01941550

Cite this article as:
Bennett, J.W., Leong, PM., Kruger, S. et al. Experientia (1986) 42: 848. doi:10.1007/BF01941550

Summary

Serial transfer of mycelial macerates of a wild type, haploid, aflatoxigenic strain ofAspergillus parasiticus in a defined liquid medium resulted in the production of three new morphological classes: a sclerotial form with high aflatoxin production, and two variant forms (fan andfluff) with lowered sporulation, no sclerotia, and attenuated levels of aflatoxin. A genetically marked diploid containing mutant markers for aflatoxin pathway intermediates yielded the same three morphological classes upon serial transfer of macerated mycelia. When these diploid variants were treated with a haploidization agent, and the phenotypes of the resultant segregants scored, a low frequency of colonies producing aflatoxin pathway intermediates was recovered. These genetic data indicate that the structural genes for the aflatoxin pathway are present but somehow attenuated in thefan andfluff strains.

Key Words

AflatoxinAspergillus parasiticussclerotiamycelial macerationstrain degeneration

Copyright information

© Birkhäuser Verlag Basel 1986

Authors and Affiliations

  • J. W. Bennett
    • 1
  • Phaik-Mooi Leong
    • 1
  • S. Kruger
    • 1
  • D. Keyes
    • 1
  1. 1.Department of BiologyTulane UniversityNew OrleansUSA
  2. 2.Molecular Biology InstituteUniversity of CaliforniaLos AngelesUSA