Experientia

, Volume 44, Issue 10, pp 882–885

Inhibitory effect of flavonoids on DNA-dependent DNA and RNA polymerases

  • K. Shinozuka
  • Y. Kikuchi
  • C. Nishino
  • A. Mori
  • S. Tawata
Short Communications

DOI: 10.1007/BF01941188

Cite this article as:
Shinozuka, K., Kikuchi, Y., Nishino, C. et al. Experientia (1988) 44: 882. doi:10.1007/BF01941188
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Summary

Flavonoids, (−)-epigallocatechin (1), myricetin (2) and quercetin (3), were investigated for inhibitory effects onE. coli DNA polymerase I and T7 bacteriophage RNA polymerase. In both DNA and RNA synthesis,1 and3 inhibited enzyme reactions by non-competitive and mixed type inhibition respecitively, with regard to template DNAs. Myricetin (2) inhibited DNA and RNA polymerase reactions by mixed type and competitive type inhibition, respectively, with template DNAs. It was suggested that2 interacts with covalently closed/circular DNA.

Key words

FlavonoidsDNA polymeraseRNA polymeraseinhibitory effect

Copyright information

© Birkhäuser Verlag Basel 1988

Authors and Affiliations

  • K. Shinozuka
    • 1
  • Y. Kikuchi
    • 1
  • C. Nishino
    • 1
  • A. Mori
    • 1
  • S. Tawata
    • 2
  1. 1.Mitsubishi Kasei Institute of Life SciencesMachida, Tokyo(Japan)
  2. 2.Department of Agricultural ChemistryUniversity of RyukyusNishihara, Okinawa(Japan)