, Volume 48, Issue 5, pp 504–508

Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties


  • J. H. Ovelgönne
    • Department of Molecular Cell BiologyState University of Utrecht
  • A. W. J. M. Bol
    • Department of Molecular Cell BiologyState University of Utrecht
  • W. C. J. Hop
    • Department of Epidemiology and BiostatisticsErasmus University
  • R. van Wijk
    • Department of Molecular Cell BiologyState University of Utrecht
Research Articles

DOI: 10.1007/BF01928175

Cite this article as:
Ovelgönne, J.H., Bol, A.W.J.M., Hop, W.C.J. et al. Experientia (1992) 48: 504. doi:10.1007/BF01928175


A previously reported2 effect of mechanically agitated dilutions of antiserum raised against IgE was investigated using the loss of metachromatic staining properties of human basophil leukocytes as a model. A series of 24 blind experiments was performed in which we determined the number of toluidine blue-stainable basophils after incubating with vortexed or non-vortexed dilutions of anti-IgE. Tenfold serial dilutions were used, in the range 1021 to 1030 (6.6×10−26 to 6.6×10−35 M anti-IgE). We found no evidence for a different effect of strongly agitated dilutions, compared to dilutions made with minimal physical agitation. In fact, in our hands no effect of extreme dilutions was shown at all. We conclude that the effect of extreme dilutions of anti-IgE, reported by Davenas et al.2, needs further clarification and that in this process the reproducibility of results between experimenters should be carefully determined.

Key words

Basophilsdegranulationextreme dilutionsin vitro testshomeopathy
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© Birkhäuser Verlag Basel 1992