Research Articles

European Journal of Plant Pathology

, Volume 102, Issue 4, pp 325-337

First online:

Comparative study of morphological, cultural and molecular markers for the characterization ofPseudocercosporella herpotrichoides isolates

  • M. L. GacAffiliated withUnité de recherches intégrées en grandes cultures, INRA
  • , F. MontfortAffiliated withUnité de recherches intégrées en grandes cultures, INRA
  • , N. CavelierAffiliated withUnité de recherches intégrées en grandes cultures, INRA
  • , A. SaillandAffiliated withLaboratoire de Biotechnologies, RhÔne-Poulenc Agro

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Nirenberg's classification system and the polymerase chain reaction (PCR) combined with restriction enzyme digestion of an amplified ribosomal DNA fragment, were compared for the characterization of sixty isolates ofPseudocercosporella herpotrichoides, from various geographical areas and with differing fungicide sensitivity. With Nirenberg's system, it was possible to identify most isolates asP. herpotrichoides var.herpotrichoides orP. herpotrichoides var.acuformis. However, identification was slow and sometimes inconclusive as overlap occurred between the two varieties for all criteria examined. Molecular markers identified two distinct types among the isolates tested and generally good correlation was found between the PCR-based assay and Nirenberg's system, but the molecular assay was more accurate and faster.

Key words

eyespot internal transcribed spacer polymerase chain reaction restriction analysis ribosomal RNA genes