, Volume 53, Issue 2, pp 129-141

The mechanism of Cl transport at the plasma membrane ofChara corallina I. Cotransport with H+

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Summary

Cl transport into cells ofChara corallina was studied in relation to that of other ions which have been proposed as cosubstrates for the Cl transport system. Although there appears to be a partial mutual dependence between K+ and Cl for transport in intact cells, this is not apparent in cells which have been perfused internally. Moreover, in intact cells, the fluxes of K+ and Cl show a large degree of independence in their responses to Cl starvation. Cl transport is electrogenic in a direction indicating the transport of excess positive charge into the cell. In the absence of any other likely counter ion, it is suggested that Cl is cotransported with H+. Response of Cl influx to internal and external pH in perfused cells is consistent with this suggestion. There appears, in addition, to be a role for ATP in transport as judged by fourfold stimulation of Cl influx in perfused cells when 1mm ATP is incorporated in the perfusion medium.