Monocarboxylic acid permeation through lipid bilayer membranes
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- Walter, A. & Gutknecht, J. J. Membrain Biol. (1984) 77: 255. doi:10.1007/BF01870573
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The membrane permeability coefficients for the homologous monocarboxylic acids, formic through hexanoic, as well as benzoic and salicylic, were determined for egg phosphatidylcholine-decane planar bilayer membranes. The permeabilities of formic, acetic and propionic acid were also determined for “solvent-free” phosphatidylethanolamine bilayers. Permeability coefficients were calculated from tracer fluxes measured under otherwise symmetrical conditions, and precautions were taken to ensure that the values were not underestimated due to unstirred layer effects. The relation between the nonionic (HA) permeability (Pm) and the hexadecane/water partition coefficient (Kp) was: logm=0.90 log Kp+0.87 (correlation coefficient=0.996). Formic acid was excluded from the analysis because its permeability was sixfold higher than predicted by the other acids. The permeabilities for “solvent-free” membranes were similar to those for decanecontaining membranes. The exceptionally high permeability of formic acid and the high correlation of the other permeabilities to the hexadecane/water partition coefficient is a pattern that conforms with other nonelectrolyte permeabilities through bilayers. Similarly, the mean incremental free energy change per methylene group (σΔG-CH2-) was −764 cal mol−1, similar to other homologous solutes in other membrane systems. However, much less negative σΔG values (−120, to −400 cal mol−1) were previously reported for fatty acids permeating bilayers and biological membranes. These values are due primarily to unstirred layer effects, metabolism and binding to membranes and other cell components.