Article

The Journal of Membrane Biology

, Volume 5, Issue 3, pp 215-224

A rapid method for the isolation of L-cell surface membranes using an aqueous two-phase polymer system

  • D. M. BrunetteAffiliated withDivision of Biological Research, Ontario Cancer InstituteDepartment of Medical Biophysics, University of Toronto
  • , J. E. TillAffiliated withDivision of Biological Research, Ontario Cancer InstituteDepartment of Medical Biophysics, University of Toronto

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Summary

A dextran-polythylene glycol aqueous two-phase system has been used to separate cell surface membranes from other cellular organelles. The surface membranes have been identified on the basis of morphology, content of Na+, K+-ATPase, and presence of surface antigen as detected by a51Cr release method. Contamination of the surface membrane preparations by smooth endoplasmic reticulum, mitochondria, and nuclei has been found to be minimal. An average of 6.5% of the total protein was found in the membrane fraction. Less than two hours is required to isolate the membrane fraction after preparation of a Dounce homogenate. Fractionation by aqueous two-phase polymer systems appears to be a rapid and effective method for the isolation of surface membranes.