The Journal of Membrane Biology

, Volume 120, Issue 1, pp 75–81

Transmembrane currents in frog olfactory cilia

  • Steven J. Kleene
  • Robert C. Gesteland

DOI: 10.1007/BF01868593

Cite this article as:
Kleene, S.J. & Gesteland, R.C. J. Membrain Biol. (1991) 120: 75. doi:10.1007/BF01868593


We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ramp to the ciliary membrane. A transient current was seen in some cells on stimulation with odorants. After excision from the cell, most of the cilia showed increased conductance in a bath containing cAMP, indicating that the cytoplasmic face of the ciliary membrane was accessible to the bath. The estimated resistance of a single cilium was surprisingly low.

Key Words

olfaction receptor electrophysiology 

Copyright information

© Springer-Verlag New York Inc. 1991

Authors and Affiliations

  • Steven J. Kleene
    • 1
  • Robert C. Gesteland
    • 1
  1. 1.Department of Anatomy and Cell BiologyUniversity of CincinnatiCincinnati