Journal of Muscle Research & Cell Motility

, Volume 11, Issue 1, pp 41–47

Deterioration induced by physiological concentration of calcium ions in skinned muscle fibres

  • N. Kasuga
  • Y. Umazume
Papers

DOI: 10.1007/BF01833324

Cite this article as:
Kasuga, N. & Umazume, Y. J Muscle Res Cell Motil (1990) 11: 41. doi:10.1007/BF01833324

Summary

The deteriorating effect of μm order of Ca2+ on skinned frog skeletal muscle fibres was studied from the view point of the digestion of proteins by calcium-activated neutral protease (CANP). Tension developed in solutions containing no MgATP (rigor solution) decreased irreversibly with the addition of Ca2+ in quantities of more than 0.1 μm. Low temperature was seen to suppress (Q10>4), and neutral pH to maximize, this decrease in tension. In rigor solution containing Ca2+, SDS electrophoresis indicated that a 95 k dalton component (α-actinin) was released from the fibre; electron micrography showed the disappearance of Z-lines. These results suggest that one of the causes for decrease in rigor tension is the proteolytic activity of CANP, and its inhibitors were shown to be quite useful in experiments on skinned fibre.

Copyright information

© Chapman and Hall Ltd. 1990

Authors and Affiliations

  • N. Kasuga
    • 1
  • Y. Umazume
    • 1
  1. 1.Department of PhysiologyJikei University School of MedicineTokyoJapan