Breast Cancer Research and Treatment

, Volume 16, Issue 3, pp 243–251

Colocalization of estrogen and progesterone receptors with an estrogenregulated heat shock protein in paraffin sections of human breast and endometrial cancer tissue

  • Daniel R. Ciocca
  • Arturo O. Stati
  • María M. Amprino de Castro
Report

DOI: 10.1007/BF01806332

Cite this article as:
Ciocca, D.R., Stati, A.O. & de Castro, M.M.A. Breast Cancer Res Tr (1990) 16: 243. doi:10.1007/BF01806332

Summary

We have studied by immunocytochemistry and monoclonal antibodies the presence and localization of estrogen receptors, progesterone receptors, and a 24-kD estrogen-regulated heat shock protein in biopsies from breast and endometrial cancer patients. Three different tissue processing protocols were used to colocalize the antigens in the same tissue sections: a) frozen sections, b) formalin fixation with routine paraffin embedding, and c) picric acid-formaldehyde (PAF) fixation with a rapid embedding in paraffin. Frozen sections showed good receptor staining but poor 24-kD protein immunoreactivity, while routine paraffin sections (with or without DNase pretreatment) were inadequate to reveal the nuclear receptor proteins at the same level seen in frozen sections. On the other hand, all three proteins could be detected satisfactorily in PAF-fixed paraffin-embedded tissue. Using this procedure we were able to visualize 24-kD protein and estrogen receptor or progesterone receptor in individual cells in paraffin sections. The study revealed that in all of the estrogen receptor positive breast and endometrial tumor samples, almost 90% of the cells expressing the cytoplasmic 24-kD protein contained estrogen receptor in the cell nucleus. In contrast, 24-kD immunoreactive cells did not express progesterone receptors in almost 40% of the progesterone receptor positive tumor samples.

Key words

estrogen receptorprogesterone receptorestrogen-regulated proteinimmunocytochemistrybreast cancerendometrial cancerheat shock protein

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Daniel R. Ciocca
    • 1
  • Arturo O. Stati
    • 1
  • María M. Amprino de Castro
    • 2
  1. 1.Unidad de Histo-Patología Endócrina, Laboratorio de Reproducción y Lactancia (LARLAC)Centro Regional de Investigaciones Científicas y Tecnológicas (CRICYT)MendozaArgentina
  2. 2.Servicio de Anatomía PatológicaHospital CentralMendozaArgentina