Cancer Immunology, Immunotherapy

, Volume 32, Issue 5, pp 303–310

Improved radioimmunotherapy of colorectal cancer xenografts using antibody mixtures against carcinoembryonic antigen and colon-specific antigen-p

  • Rosalyn D. Blumenthal
  • Rina Kashi
  • Richard Stephens
  • Robert M. Sharkey
  • David M. Goldenberg
Original articles

DOI: 10.1007/BF01789048

Cite this article as:
Blumenthal, R.D., Kashi, R., Stephens, R. et al. Cancer Immunol Immunother (1991) 32: 303. doi:10.1007/BF01789048

Summary

Radioimmunotherapy of GW-39 human colonic tumor xenografts grown in the hamster cheek pouch with131I-labeled NP-4 anti-(carcinoembryonic antigen) (CEA) and131I-labeled Mu-9 anti-(color-specific antigen-p) (CSAp) murine monoclonal antibodies, administered in combination, was more effective than using either antibody alone for tumor masses less than 0.5 cm3 in size. The antibody mixture had no therapeutic advantage for larger tumors. Therapeutic efficacy was determined by measuring the change in tumor size over time, quantifying the absolute number of tumors responding to radioantibody therapy, and determining the percentage growth inhibition of each treatment at various times after radioantibody administration. Several mechanisms are discussed to explain the improved tumoricidal effect of the antibody mixture noted in this model system, such as (a) the possibility that an antibody mixture could target a greater number of tumor cells, (b) the potential for antibody mixtures to provide better tumor distribution and (c) the possibility that antibodies administered in combination can increase the magnitude of tumor uptake of individual radioantibodies, thereby resulting in a greater radiation dose delivered to the tumor.

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Rosalyn D. Blumenthal
    • 1
  • Rina Kashi
    • 1
  • Richard Stephens
    • 1
  • Robert M. Sharkey
    • 1
  • David M. Goldenberg
    • 1
  1. 1.Center for Molecular Medicine and ImmunologyNewarkUSA