Mechanical properties of mammalian single smooth muscle cells I. A low cost large range microforce transducer
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A transducer has been developed for measuring the minute forces generated during isometric contractions (1.0–10.0μN) of single smooth muscle cells from the pig urinary bladder and the human uterus. In addition to its high sensitivity, resolution and stability (100 mVμN−1, <0.1μN and <2.0μN h−1), the transducer features a very wide range (100–140μN) with good linearity, enabling measurement of contractions as well as passive force-length characteristics within one uninterrupted measurement session. Since the transducer features an independent and interchangeable force to displacement conversion system, different force ranges can be realized by inserting force conversion systems with different compliances.
- Brehm, R. &Grootel, P. Van (1985) Toepassing van glaskeramiek in een precisie-meetapparaat.Nederlandse Vereniging voor Glastechniek informatie,2, 2–3.
- Canaday, P.G. &Fay, F. S. (1976) An ultrasensitive isometric force transducer for single smooth muscle cell mechanics.J. Appl. Physiol. 40, 243–6.
- Dijk, A. M. Van, Wieringa, P. A., Meer, M. Van Der &Laird, J. D. (1984) Mechanics of resting isolated single vascular smooth muscle cells from bovine coronairy artery.Am. J. Physiol. 246, C277–87.
- Glerum, J. J., Mastrigt, R. Van, (1990) Mechanical properties of mammalian single smooth muscle cells, II: Evaluation of a modified technique for attachment of cells to the measurement apparatus.J. Musc. Res. Cell Motil. 11, 338–343.
- Ishii, N. &Takahashi, K. (1982) Length-tension relation of single smooth muscle cells isolated from the pedal retractor muscle ofMytilus edulis.J. Musc. Res. Cell Mot. 3, 25–38.
- Ishii, N., Simpson, A. W. M. &Ashley, C. C. (1988) Intracellular free calcium ([Ca2+]i) and the ‘catch’ contraction in isolated molluscan smooth-muscle (ABRM) cells. (abstract)J. Musc. Res. Cell Mot. 9, 463.
- Iwazumi, T. (1982) High performance instrument for myofibrillar mechanics, (abstract)Bioph. J. 37, 357a.
- McLaughlin, R. J. (1977) Systematic design of cantilever beams for muscle research.J. Appl. Physiol. 42, 786–94.
- Meiss, R. A. (1971) An isometric muscle force transducer.J. Appl. Physiol. 30, 158–60.
- Meiss, R. A. (1974) A versatile transducer system for mechanical studies of muscle.J. Appl. Physiol. 37, 459–63.
- Minns, H. G. (1971) A voltage-controlled force generator for calibrating sensitive transducers.J. Appl. Physiol. 30, 895–6.
- Minns, H. G. &Franz, G. N. (1972) A low-drift transducer for small forces.J. Appl Physiol 33, 529–31.
- Tung, L. (1986) An ultrasensitive transducer for measurement of isometric contractile force from single heart cells.Pflügers Arch. 407, 109–15.
- Warshaw, D. M. &Fay, F. S. (1983) Cross-bridge elasticity in single smooth muscle cells.J. Gen. Physiol. 82, 157–99.
- Wieringa, P. A., Meer, M. Van Der, Dijk, A. M. Van &Laird, J. D. (1984) Sensitive force transducer system for mechanical studies of single isolated vascular smooth muscle cells.Med. & Biol. Eng. & Comp. 22, 130–7.
- Mechanical properties of mammalian single smooth muscle cells I. A low cost large range microforce transducer
Journal of Muscle Research & Cell Motility
Volume 11, Issue 4 , pp 331-337
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