Article

Inflammation Research

, Volume 44, Issue 10, pp 434-437

First online:

Zonal differences in nitric oxide synthesis by bovine chrondrocytes exposed to interleukin-1

  • K. FukudaAffiliated withDepartment of Orthopaedic Surgery, Kinki University School of Medicine
  • , F. KumanoAffiliated withDepartment of Orthopaedic Surgery, Kinki University School of Medicine
  • , M. TakayamaAffiliated withDepartment of Orthopaedic Surgery, Kinki University School of Medicine
  • , M. SaitoAffiliated withDepartment of Orthopaedic Surgery, Kinki University School of Medicine
  • , K. OtaniAffiliated withDepartment of Orthopaedic Surgery, Kinki University School of Medicine
  • , S. TanakaAffiliated withDepartment of Orthopaedic Surgery, Kinki University School of Medicine

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Abstract

To determine the role of nitric oxide (NO) in the inhibition of aggrecan synthesis, we measured levels of NO produced by bovine chondrocytes from different layers of articular cartilage in the presence of interleukin-1 (IL-1). Chondrocytes from the superficial layer showed a large increase in NO synthesis in response to IL-1. Although chondrocytes from the deep layer also produced NO in response to IL-1, the amount was less than that from the superficial layer. Enhanced NO production evoked by IL-1 was accompanied by a significant inhibition of aggrecan synthesis. These data suggest that chondrocytes in both superficial and deep layer of articular cartilage inhibit aggrecan synthesis with IL-1 via NO production. In addition, superficial layer cells respond to lower amounts of IL-1 with respect to NO-production and inhibition of proteoglycan synthesis.

Keywords

Cartilage Arthritis Nitric oxide Interleukin-1