Clinical & Experimental Metastasis

, Volume 12, Issue 6, pp 357–367

Mammary carcinoma cell lines of high and low metastatic potential differ not in extravasation but in subsequent migration and growth

  • Vincent L. Morris
  • Sahadia Koop
  • Ian C. MacDonald
  • Eric E. Schmidt
  • Marsha Grattan
  • Dean Percy
  • Ann F. Chambers
  • Alan C. Groom
Research Papers

DOI: 10.1007/BF01755879

Cite this article as:
Morris, V.L., Koop, S., MacDonald, I.C. et al. Clin Exp Metast (1994) 12: 357. doi:10.1007/BF01755879

Abstract

We examined the extravasation and subsequent migration and growth of murine mammary tumor cell lines (D2A1 and D2.OR) which differ in their metastatic ability in lung and liver, invasivenessin vitroand expression of the cysteine proteinase cathepsin L. In light of the differences in invasiveness and cathepsin L expression, we hypothesized that during hematogenous metastasis the two cell lines would differ primarily in their ability to extravasate. We usedin vivovideomicroscopy of mouse liver and chick embryo chorioallantoic membrane to examine the process and timing of extravasation and subsequent steps in metastasis for these cell lines. In contrast to our expectations, no differences were found between the cell lines in either the timing or mechanism of extravasation, at least 95% of cells having extravasated by 3 days after injection. However, after extravasation, the more metastatic and invasive D2A1 cells showed a greater ability to migrate to sites which favor tumor growth and to replicate to form micrometastases. These studies point to post-extravasation events (migration and growth) as being critical in metastasis formation.

Keywords

cancer cell migration chorioallantoic membrane extravasation liver mammary carcinoma lines tumor growth 

Copyright information

© Rapid Communications of Oxford Ltd 1994

Authors and Affiliations

  • Vincent L. Morris
    • 1
    • 2
  • Sahadia Koop
    • 3
  • Ian C. MacDonald
    • 3
  • Eric E. Schmidt
    • 3
  • Marsha Grattan
    • 1
  • Dean Percy
    • 4
  • Ann F. Chambers
    • 1
    • 2
    • 3
    • 5
  • Alan C. Groom
    • 3
    • 6
  1. 1.Department of Microbiology and ImmunologyUniversity of Western OntarioLondonCanada
  2. 2.Department of OncologyUniversity of Western OntarioLondon
  3. 3.Department of Medical BiophysicsUniversity of Western OntarioLondon
  4. 4.Department of PathologyUniversity of GuelphGuelph
  5. 5.London Regional Cancer CentreLondon
  6. 6.John P. Robarts Research InstituteLondonCanada