Journal of Molecular Evolution

, Volume 7, Issue 4, pp 325–329

5-methylcytosine content in the vertebrate deoxyribonucleic acids: Species specificity

  • R. M. Kothari
  • V. Shankar
Letter to the Editor

DOI: 10.1007/BF01743628

Cite this article as:
Kothari, R.M. & Shankar, V. J Mol Evol (1976) 7: 325. doi:10.1007/BF01743628


RNA-free native DNA samples, isolated by four methods, from different vertebrate tissues and species, were hydrolyzed chemically and enzymatically and analyzed by paper chromatography to estimate the base composition. It was noted that (i) all the DNA preparations analyzed contained 5-methylcytosine, (ii) on the basis of mole percent of 5-methylcytosine, the composition of DNA varied in different species, but not so much in different tissues of the same species, (iii) the method of DNA hydrolysis, but not the method of deproteinization, affected the mole percent of 5-methylcytosine, and (iv) no 5-hydroxymethylcytosine (5-HMC) was detected in any of the DNA preparations analyzed.

Key words

Vertebrate TissueDNA Base AnalysisPaper Chromatography5-Methylcytosine Content

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • R. M. Kothari
    • 1
  • V. Shankar
    • 1
  1. 1.Department of Zoology and Department of ChemistryUniversity of PoonaPoona-7India
  2. 2.Department of Environmental BiosciencesNaval Medical Research Inst., National Naval Medical CenterBethesdaUSA
  3. 3.Department of BiochemistryCollege of Physicians and Surgeons Columbia UniversityNew YorkUSA